|
Bangladesh
Journal of Biochemistry. Vol. 4, No. 1&2, 1-11, 1998
ISOLATION
AND CHARACTERIZATION OF A THIRD RIBOSOME INACTIVATING PROTEIN, LUFFIN-C, FROM
SPONGE GOURD (LUFFA CYLINDRICA) SEEDS – AN IMPROVED METHOD FOR
PURIFICATION OF LUFFIN VARIANTS
M. Rafiqul ISLAM,
Present address: Department of Chemistry, Northwest Missouri State University,
800 University Drive, Maryville, MO 64468, USA.
Keiichi WATANABE,
Present address: Department of Applied Biological Science, Saga University, Saga
840, Japan.
(Laboratory of Protein Chemistry
and Engineering, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan.)
Received January 15,
1998
Abstract
Previously
two ribosome-inactivating proteins, named luffin-a and –b,
were isolated from the seeds of luffa cylindrica (Kamensono
et al, 1988). A new ribosome-inactivating protein, which we named
luffin-c has been purified to homogeneity from a salt-acid extract
of sponge gourd seeds by chromatography on an AF-Blue Toyopearl column
followed by a fast protein liquid chromatography (FPLC) on a Mono-S
column. Luffin –c, which has a similar molecular mass (28 kDa) and
isoelectric point (>10) as those of luffin-a and –b, showed a distinct
elution time in FPLC Mono-S column. In addition to isolate luffin-c,
this rapid purification method improved
the isolation of both luffin-a and –b. Amino acid and carbohydrate
composition analyses showed that is similar to luffin-a in glycine,
alanine, isoleucine and carbohydrate contents, while it is similar
to luffin-b in threonine, proline and methionine contents, but its
serine content is unusually low than the serine contents of both luffin-a
and –b.Comparison of N-terminal sequences of luffin –a, -b
and –c indicated although they are homologous proteins, luffin-c has
an extension of two and three residues from the N-terminals of luffin-b
and –a, respectively. The biological activities of luffin-c, measured
by inhibition of protein synthesis in rabbit reticulocyte lysates
and by inactivation of rat liver ribosomes, were similar to those
of luffin-a. However, both luffin-a and –c were found to be 3-4 times
stronger in those activities than luffin-b.
top
Bangladesh
Journal of Biochemistry. Vol. 4, No. 1&2, 13-21, 1998
VARIATION
OF THE BODY COMPOSITION OF HILSHA (TENUALOSA ILISHA) DURING GONAD
DEVELOPMENT
Syeda
Shamim JAHAN*, Mohammad MUSLEMUDDIN, Syed Fazl-e- RUBBI and Benedict
GOMEZ1
Institute of Food Science and Technology, BCSIR,Qudrat-e-Khuda Road.,
Dhanmondi, Dhaka 1205, Bangladesh.
*Corresponding author.
1Present address:
Department of Biochemistry, Dhaka University, Dhaka.
(Received
May 10, 1998)
Abstract
The proximate
composition of hilsha fish (Tenualosa
ilisha) varies with the variation of the physiological condition
of the fish. The sequences of energy mobilization from net loss of
muscle calorie and gain in roe and liver calories were calculated
during gonad development. Protein and fat contents in the whole muscle
of hilsha decreased with increase in the severity of depletion. The
rates of change of these nutrients were different at different
anatomical portions like dorsal, ventral, tail and dark muscle
of hilsha. The protein and fat contents of the gonad increased and
moisture content decreased with the increase of the size of the roe.
The fat content in the liver also increased with no appreciable change
in protein content. In a healthy fish maximum amount of energy comes
from its muscle (98.4% of total energy) and the roe contributes a
very small amount (1.2%). On the other hand, in a depleted fish with
matured gonad only 41.7% of the total energy comes from the muscles, while the roe contributes 56.9%. With the progress
of gonad development total energy reserve of the body decreases, due
mainly to loss of fat calorie.
top
Bangladesh
Journal of Biochemistry. Vol. 4, No. 1&2, 23-31, 1998
CONFORMATION AND SURFACE PROPERTIES OF DEAMIDATED
GLUTEN OF GERMINATING WHEAT
Ranjit Kumar SHAHA 1*,
and Akio KATO2
1Department of Biochemsitry,
University of Rajshahi, Rajshahi-6205, Bangladesh and 2Department of
Biochemistry, Yamaguchi University, Japan.
*Corresponding author.
(Received
May14, 1998)
Abstract
The surface properties
of deamidated gluten were investigated with respect to their conformational
changes. The helix content of gluten decreased curvilinearly with
its decrease of deamidation. The surface tension decresed in proportion
to the degree of deamidation. On the other
hand, the surface hydrophobicity of gluten increased remarkably
in proportion to the degree of deamidation. The emulsifying properties
of gluten were improved greatly by deamidation, correlating linearly
with the surface hydrophobicity. From these results, the relationship
between the conformational
changes and functional properties of deamidated gluten are discussed.
top
Bangladesh
Journal of Biochemistry. Vol. 4, No. 1&2, 33-41, 1998
DEVELOPMENT OF LOW-COST MEDIA FOR LARGE SCALE
PRODUCTION OF BACILLUS SPHAERICUS
Mahmud
HOSSAIN, Nawaz KHAN, M. JAHANGIR, Anwar HOSSAIN, K. A. RAHIM and Mustafizur
RAHMAN.
Department of Biochemistry, Dhaka University, Dhaka-1000, Bangladesh
(Received
June 16,
1998)
Abstract
Three media
formulated from bovine blood serum, soya meal and fish meal in
combination with mineral salts were assessed for the growth
of Bacillus sphaericus strains 1593 and SI-I, their sporulation
and their toxicity against mosquito larvae. Freeze dried powders of
these bacterial strains were found to be active against the larvae
of Culex quinquefascitatus (CQF). All the media prepared from indigenous
sources performed better than the commercially available chess medium.
The highest number of viable cells and spores -8.2×108
and 8.1×108 respectively for Bacillus sphaericus (1593)
and 1.2×109and
1.4×109
respectively for Bacillus sphaericus SI-1-was found in bovine
serum medium. For CQF, the LC50
value of Bacillus sphaericus SI-1
strain (1µg/ml) was reached in 18 hrs. Both Bacillus
sphaericus 1593 and Bacillus sphaericus SI-1 strains were tried against
the larvae of Aedes also. Only the latter one was found to be active
against this larvae, but the activity was extremely poor. Addition
of Ca++ in the medium increased the potency of the strains
2-3 times.
top
Bangladesh
Journal of Biochemistry. Vol. 4, No. 1&2, 43-54,
1998
A COMPARATIVE STUDY ON CHARACTERISTICS OF BOILED AND
NON-BOILED TYPES OF RICE BRAN AND THEIR OILS
M. Mokhlesur RAHMAN,
M. Maksud ALI and Nurul ABSAR*
Department of Biochemistry, University of Rajshahi, Rajshahi, Bangladesh
(Received October
26,1998)
Abstract
Ten samples of
boiled and non-boiled types rice bran (BR-14
variety) collected from different rice growing areas of Bangladesh,
have been examined for their total lipid (oil), non-lipid components
and physico-chemical properties of this total lipid. Minerals content
such as calcium, iron and phosphorus were present in good proportion
in both boiled and non-boiled types rice bran. Of the major energy
producing nutrients, boiled rice bran contained higher amounts of
carbohydrates and fat
but non-boiled bran contained higher amount of protein. The total
lipid varied from 16.00-18.68%, ash 8.45-9.70%, protein 14.04-15.10%
and crude fibre ranged from 4.30-8.92%. Oils extracted from fresh
rice brans contained FFA usually between 2.16-2.52%. Total lipid extracts
were fractionated into lipid classes by a combination of column and
thin layer chromatography. The triglycerides varied from 91.335 to
94.20%, partial glycerides from 1.6% to 4.2% and free sterol from
0.2% to 0.5%. Glycolipid and phospholipid amounted to 0.6%-1.2% and
0.2%-0.5% respectively. The fatty acid compositon of the oils showed
that rice bran oil contained 15.00-16.90% of palmitic acid, 2.42-4.20%
of steric acid, 45.30-46.62% of oleic acid and 30.28-33.55% of linoleic
acid as major fatty acid.
top
Bangladesh
Journal of Biochemistry. Vol. 4, No. 1&2, 55-70, 1998
BIOCHEMICAL CHARACTERISTICS OF SOME EDIBLE AND
NON-EDIBLE FATS AND OILS OF BANGLADESH.
Nazibur RAHMAN and
Harun K. M. YUSUF*
Department of Biochemsitry, University of Dhaka.
(Received November 30,
1998)
Abstract
A comparative
study was made on some characteristics of edible and non-edible fats
and oils available in Bangladesh. Comparison was made between the
purified and non-purified forms of the same edible fats and
oils. The parameters based on which the nutritional value of
all the fats and oils were determined were iodine value, saponification
value, acid value and fatty acid composition. In the case of edible
oils, soybean (Postman) had the highest iodine value (149.49) whereas
coconut (aromatic) had the lowest iodine value (11.32). Coconut (aromatic)
had the highest saponification value (268) whereas ghee (home-made)
had the lowest saponification value (211). Butter had the highest
acid value (12) whereas palm (Dhaka) had the lowest acid value (0.17).
Chromatographic analysis of fatty acids showed that soybean (USA)
contained the % highest proportion of
ω-6 fatty acids (54) whereas
ghee of the open market contained the lowest (0.89). It was remarkable
that coconut (aromatic), ghee (home-made), butter, dalda, mustard
of the open market and mustard of ghani did not contain the ω-6 fatty acids. Palm from Chapai Nawabganj,
palm from Dhaka, coconut (open market), coconut (aromatic), ghee(open
market), ghee (home-made) butter and dalda did not contain
any kind of ω-6 fatty acids. In the case
of non-edible oils, percentage of iodine value was highest in tisi
(137.35), whereas chamily had the lowest (1.35). A remarkable feature
was that tisi contained such an enourmous proportion of 18:3 (ω-3) fatty acid (38.9) that could not
be found normally in any kind of edible oil.
top
Bangladesh
Journal of Biochemistry. Vol. 4, No. 1&2, 71-80, 1998
NEEDLE TYPE OXYGEN ELECTRODE FOR THE DETECTION OF
OXYGEN TENSION IN TISSUES
Sayed AHMED,
Department of Laboratory Medicine, Institute of Child and Mother Health, Matuail,
Dhaka-1362, Bangladesh.
M. Anwar HOSSAIN,
Department of Biochemistry, University of Dhaka, Dhaka-1000, Bangladesh.
and Pankaj M. VADAGAMA
Section of Clinical Biochemistry, Department of Medicine, University of
Manchester. UK.
(Received September 2,
1998)
Abstract
After a bony fracture
or injury, blood vessels are usually damaged and the tissues distal
to the injury suffer from ischemia. As a consequence, the bony tissue
gradually becomes necrosed and ultimately dies. It is very difficult
to pinpoint the area of necrosis, which is essential for the further
surgical procedure. The needle type oxygen electrod developed in our
laboratory has the potential for the detection of dead tissues. The
ability of these electrodes to detect oxygen in buffer sample and
its effects on exposure to whole blood and serum are presented.
top
Bangladesh
Journal of Biochemistry. Vol. 4, No. 1&2, 81-92, 1998
A COMPARATIVE STUDY ON SERUM PROTEINS IN GOITER
PATIENTS AND NORMAL INDIVIDUALS
M. Abdulla HARUN,
Mohammad Rezaul KARIM*, Nurul ABSAR and M. SHAHAJAHAN
Department of Biochemistry, University of Rajshahi, Rajshahi-6205,
Bangladesh.
(Received April 1,
1998)
Abstract
Abnormal serum
protein fractions for goiter patient were examined by determining
serum total protein, serum albumin, globulin and albumin/globulin
ration (A/G) by SDS-polyacrylamide gel electrophoresis. γ
-globulin was purified and characterized in both cases of goiter and
normal human. The amount of serum total protein increased remarkably
(29%)in goiter patients. This increase was mainly due to an abnormal
rise in the amount of globulin. The amount of serum albumin was found
in the normal ranges between 4.3 and 4.6. α1,
α2, γ-globulin increased but β -globulin decreased. γ-globulin was significantly (59%)
increased in goiter. The apparent molecular weight of IgG as estimated
by SDS-PAGE was 1,58,325. The purified IgG was found to be nonspecific
in precipitation by serum of goiter patient (antigen) and in hemagglutination
of rat red blood cells.
|
